Lionel Messi, Neymar, Dani Alves and Javier Mascherano will not play against Fiorentina on Sunday.
The four stars will remain in Barcelona to continue their pre-season, taking a training session on the day the team travel to Italy to play.
On Thursday the two Argentines turned up by surprise and took their medicals, despite having a few more days of holiday left.
It's likely that the four will all play some part in the Joan Gamper Trophy next week against Roma, August 5 at the Camp Nou (22h CET).
The objective is for the four to arrive at a good level of fitness in time to compete in the Super Cups.